Regulatory

Part:BBa_K175046

Designed by: Daniel Solis Escalante   Group: iGEM09_TUDelft   (2009-10-12)

Plasmid 1 for Transcriptional Cascade subproject TU Delft iGEM 09

This part is the plasmid 1 of the transcriptional cascade subproject of TU Delft iGEM. It generates the transcriptional regulator cI and RFP under the control of PTet promoter. This part in combination with the plasmid 2 (K175047) will lead to a delay in the expression of GFP after IPTG induction. The detailed description of the behavior and characterization of this part can be seen [http://2009.igem.org/Team:TUDelft/Results here]. And the characterization of the delay device can be seen here.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1436
    Illegal AgeI site found at 1548
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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